Fig. 2

Expression and release of Hsp70 following hyperthermia treatment. One and a half million CT26 cells were heated to 42 °C for 30 min using LabEHY or a water bath (control). a After 24 h of incubation, cells were harvested for western blot analysis of Hsp70 or HMGB1 protein in lysates of CT26 (control) (37 °C), a water bath (control) (42 °C) or mEHT. β-actin was used as an internal control. b After 24 h of incubation, supernatants were harvested and concentration of Hsp70 was measured using ELISA (n = 3). Data are presented as mean +/− SD from three independent experiments. * indicates p < 0.05