Fig. 3
From: Enhancement of Cetuximab-Induced Radiosensitization by JAK-1 Inhibition
Immunoblot analyses revealed that cells treated with cetuximab (5 μg/ml) and the combination of cetuximab and JAK1i (1 μM) showed reductions in p-EGFr. Additionally, cells treated with JAK1i (1 μM) alone showed increased p-EGFr. Parental UM-SCC-5 cells, STAT-3 knockdown cells (STAT3-2.4) and control transfected cells (NEG 4.17) were treated with cetuximab (5 μg/ml) and/or JAK1i (1 μM) for 24 h (with and without 5 mins of exposure to EGF [60 mg/ml] at the end of the 24 h) and assessed for p-EGFr, EGFr and GAPDH as described in the methods section