Fig. 6

RT-qPCR validation and immunohistochemical evaluation of DNMT3A in MYC translocation-positive and MYC translocation-negative BL primary tumors. a Quantitative-RT-PCR The expression of DNMT3A was analysed at the mRNA level by RT-qPCR. As for DNMT1, DNMT3A resulted up-regulated in cases lacking the translocation; (b-c) Immunohistochemistry. In the exemplifying MYC translocation-positive case (b), the staining is shown in 40 % of neoplastic cells in contrast to the MYC translocation-negative one (c), in which about 60 % of cells are positive. b-c: DNMT3A stain. O.M: 20x