Fig. 5

RT-qPCR validation and immunohistochemical evaluation of DNMT1 in MYC translocation-positive and MYC translocation-negative BL primary tumors. a Quantitative-RT-PCR. The expression of DNMT1 was analysed at the mRNA level by RT-qPCR. The results show up-regulation of DNMT1 in cases lacking the translocation; (b-c) Immunohistochemistry. In the exemplifying MYC translocation-positive case (b), the staining is present in about 30 % of neoplastic cells, in contrast to the MYC translocation-negative one (c), in which the positivity is depicted in about 80 % of cells. b-c: DNMT1 stain. O.M: 20x