Fig. 5

Abnormal ducts induced in fully adult, virgin glands through activation of NF-κB in mammary epithelium. 16 week old adult, virgin IKMV and control females, which were previously untreated, were given dox (1 g/L) for 10 days prior to sacrifice. A subtle enlargement of ducts can be seen in haematoxylin stained whole mounts (left panels). The phenotype is more apparent in H&E stained sections (100 μm calibration bar) (right panels). IKMV ducts are filled with cells and significantly larger than the controls. Size of ducts is quantified below images (n = 3 control and n = 3 IKMV glands, total of 64 ducts were measured; ***p = 0.0003)