Fig. 3

Short term activation of NF-κB in mammary epithelium leads to ducts with filled lumens. Intact 6 week old virgin IKMV and control littermates were dox-treated (2 g/L) for 3 days prior to sacrifice. a Haematoxylin stained whole mounts of control and IKMV glands reveal changes in IKMV ducts. In H&E stained sections (below), we observed a complete occlusion of IKMV ducts throughout the gland. b Increased size of individual IKMV ducts is apparent in 20X images with calibration bars (150 μm). Multiple measurements of duct area across samples are quantified at right (n = 3 control, n = 3 IKMV glands, total of 65 individual ducts were measured; *** p < 0.001). c Double transgenic IKMV virgin females were kept on normal water at the 6 week virgin time point and collected 3 days later along with the dox-treated cohort. Images of H&E stained mammary tissue show ducts of untreated controls have normal morphology, with no lumen-filling or hyperplastic growth (10X magnification at left, 20X at right)