Fig. 3

Comparison between BSP, NBSP and cloning-based methods in the analysis of miR-200c/miR-141 locus of MDA-MB-231 breast cancer cell line. a miR-200c/miR-141 locus PCR of bisulfite treated DNA obtained from MDA-MB-231. Lane M, 100 bp size marker. NTC, no template control. b Representative sequencing chromatogram of 6 CpG (highlighted by gray arrows; left panel) and of the reverse sequence of Tail2 of miR-200c/miR-141 amplicon (right panel). C and T used to calculate the NF are highlighted by black and white arrows, respectively. c The methylation percentages of each CpG obtained from the cloning-based method (22 clones sequenced, white columns), BSP (black columns) and NBSP (gray columns) are reported. BSP and NBSP were performed on three MDA-MB-231 samples. Bars correspond to standard deviation