Fig. 9

Rho Activity assay in primary mouse mammary epithelial cells. a Representative western blot showing the activation of RhoA in primary mammary epithelial cells plus or minus serum addition as described in the materials and methods section. b Bar graph showing the active RhoA levels determined by normalizing the amount of RBD-bound Rho to the total RhoA in whole cell lysates. Data is represented as the mean ± SEM from a total of 6 independent experiments. Statistical analyses were performed using student T test. *p < 0.05; **p < 0.01. [WT - FBS vs WT+FBS, p = 0.04; Dlc1^gt/+-FBS vs Dlc1^gt/++FBS, p = 0.022; WT-FBS vs Dlc1^gt/+ -FBS, p = 0.021; WT+FBS vs Dlc1^gt/++FBS, p = 0.0085]