Fig. 6

Mammary epithelial cells from Dlc1^gt/+ mice show defects in acinar lumen formation when grown in Matrigel cultures. a Representative images of acinar structures formed from mammary epithelial cells from 10 week old wild type (top) and Dlc1^gt/+ (lower) mice grown in 3D Matrigel cultures. Acini are shown at day 12 after plating. Acinar structures were stained with To-Pro-3 and antibodies against α6 integrin (left), or β-catenin (right). b Acinar structures were stained with E-Cadherin and To-Pro-3 from 10 week old wild type (top) and Dlc1^gt/+ (lower). Scale bar, 50 μm. Serial confocal images were taken using Z-stacking function through the middle of the acini. c Quantification of acini showing filled lumens. Data is the mean ± SD from a total number of 5 different glands (N = 5) from 5 different mice for each. **p < 0.001