Fig. 5

Histological sections of mouse mammary glands stained with cytokeratin lineage markers, cleaved caspase-3 and Ki67. a–b Immunostaining of the mouse mammary glands with lineage markers cytokeratins 14 (Basal/myoepithelial) and 18 (Luminal) from 10-week old WT mice (a) and Dlc1^gt/+ mice (b). Cleaved caspase-3 staining in 10-week old Dlc1^gt/+ (d) and WT mice (c). Ki67 staining of mouse mammary glands from WT (e) and Dlc1^gt/+ (f). The orange arrows indicate cells with Ki67 positive nuclei. The bar graph is the percentage of Ki67 positive cells (g). Data represent the mean ± SEM from a total number of 3 different glands (N = 3) from 3 different mice for each group p = 0.2. Scale bar 50 μm