Fig. 1

2-deoxyglucose inhibits PC3 prostate cancer cell growth and inhibits autophagy. PC3 cells were incubated with different concentrations of 2-deoxyglucose (2DG). a Cell growth rate was measured by counting the cell numbers at the indicated time points for 3 days. Data are represented as means ± SD. ***P < 0.001, control versus 5 mM 2DG; ***P < 0.001, control versus 10 mM 2DG; ***P < 0.001, control versus 20 mM 2DG. (b) After 2DG treatment for 48 h, autophagy levels were detected using western blotting analysis. p62 was used as an autophagy substrate and LC3B showed autophagy levels. (c) The level of autophagy was confirmed with confocal microscopy. Blue, nucleus; Green, LC3B; Red, p62