Fig. 3

Validation of potential markers for intrinsic cisplatin refractoriness by q-RT-PCR. a Expression levels of FMN1 in cisplatin-surviving clones from all three biological replicates were determined by microarray analysis (left panel) and q-RT-PCR (right panel). b Relative mRNA expression of DKK1, XRCC2 and LGALS9 was determined by q-RT-PCR from replicates 1 and 3, respectively. For all mRNA analyses, 18S rRNA was used to control for loading differences among the samples. The relative expression of each gene in cisplatin-surviving NSCLC residual clones is given as fold change relative to untreated NSCLC clones which are set to one