Fig. 1

Colony formation assay after treatment of U-1810 cells with cisplatin. a Schematic outline of the experiments. NSCLC U-1810 cells were either untreated or cisplatin-treated for 1 h. Cells were then allowed to form colonies over 9 days in drug-free media. One set of dishes was stained with 0.5 % crystal violet and the number of colonies was counted. Other sets of dishes were used for extraction of total RNA for gene expression profiling. Ingenuity Pathway Analysis software was used to identify altered cellular networks based on differential gene expression (>1.5 fold up- or down-regulated) relative to untreated cells. A number of genes were selected for further validation by q-RT-PCR. b Relative clonogenic survival of NSCLC U-1810 cells after treatment with 2.5-20 μM cisplatin. For each concentration, three biological replicates were performed where the survival of untreated cells was set to one. c Total cell number was calculated using a haemocytometer for the surviving clones after 10 μM cisplatin. ***; p<0.005