Fig. 5

siRNA knock-down of Notch1 reduces basal expression of MRP1 in T47Ds but not HB2s. Cells were transiently transfected with control or Notch1, 2, 3 or 4-targetted siRNAs for 72 h. Expression of Notch1 andABCC1/MRP1 was quantified using qPCR and is presented relative to siScrambled (control) transfected levels. Mean levels from 3 independent siRNAs to each target are presented and results show mean of 2 biological repeats (error bars represent SEM). T-tests were used to determine significant changes, * p < 0.05, ns = not significant