Fig. 8
Re-challenge study and re-treatment assay. a Schematic representation of the performed protocols. The mice that had survived for 200 days in the experiment described in Fig. 6 were challenged with 10.000 4T1 cells implanted in the forth left mammary fat pad (opposite to the site where the first tumor was implanted). Ten days later tumors were already palpable and some of these animals were left without further treatment (Sur) or underwent tumor surgery 16 days later (Sur + S-16), or received SFV-IL-12 (2×108 vp in 50 μl PBS) at day 10 and LVR01 (2×107 cfu in 50 μl PBS) at day 13 (Sur + SFV-IL-12 + LVR01), or received this last treatment followed by surgery (Sur + SFV-IL-12 + LVR01 + S-16). A healthy and untreated group of animals with similar age were equally inoculated with 10.000 4T1 cells and left without further treatment (Control) or underwent surgery (Control + S-16). b Kaplan–Meier plot shows the survival rate of the indicated groups of mice (n = 6-9). Survival days were counted since the second (re-challenged) 4T1 inoculation. p< 0.01 (**); p< 0.001 (***)