Fig. 3
From: Patient-derived heavy chain antibody targets cell surface HSP90 on breast tumors
Identification of target antigens of HCAb1 and HCAb2. a and b Immunoprecipitation of respective target antigens by HCAb1 and HCAb2. 20 μg of HCAb1 and HCAb2 were used to immunoprecipitate the target antigens from 1 % Triton X-100 (a) or 1 % Triton X-100 + 0.1 % SDS (b) MDA-MB-231 lysates. Immunoprecipitated proteins were run on a reducing SDS-PAGE gel and proteins in the gel were stained with SYPRO® Ruby stain. * indicates specific band for HCAb1 (a) and HCAb2 (b)