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Table 1 pMEC were sorted to remove fibroblasts (CD140-), and some selected for expression of CD49f or tdTomato, and then transduced at various passages (PT) with one of the lentiviral constructs PGK-Tantigen (PGKT), CMV-tdTomato (CMVT) or PGKT-CMVT (PTCT)

From: Neoplastic transformation of porcine mammary epithelial cells in vitro and tumor formation in vivo

Cell line FACS PT In vitro morphology Number Age PI Cells (#) Matrix Site E + P Tumor diameter (mm) Weeks carried in vivo characteristics
ss071712 PGKT CD140- 1 Cobblestone, no foci 3 67d 6 1×105 Hydrogel MG No N/a 16.7 N/a
ss071712 CMVT CD140- 6 1×105 Hydrogel MG No N/a 16.7
ss071712 PGKT CD140- 1 7 74d 6 1×105 Hydrogel MG No N/a 15.7 N/a
ss071712 CMVT CD140- 6 1×105 Hydrogel MG No N/a 15.7
27-3 PTCT CD140-tdTomato+ 1 Foci, radial outgrowth 4 30d 7 5×105 Hydrogel MG No <1 mm 19.5 Normal ductal epithelium
27-3 PTCT CD140-tdTomato+ 7 5×105 Hydrogel SC (Rear) No N/a 19.5 N/a
28-3 PTCT CD140- 1 Elongated, some foci 4 30d 10 5×105 Hydrogel MG No 15.3 ± 1.5 18.5 Fibrosis, vimentin positive
28-3 PTCT CD140- 10 5×105 Hydrogel SC (Rear) No 12.3 ± 1.1 18.5
27-1 PTCT CD140-tdTomato+ 1 Cobblestone, no foci 4 30d 11 5×105 Hydrogel MG No N/a 36.4 N/a
27-1 PTCT CD140-tdTomato+ 11 5×105 Hydrogel SC (Rear) No N/a 36.4
28-6 PTCT CD140-tdTomato+ 1 Foci, rapid proliferation 4 30d 11 5×105 Hydrogel MG No <1 mm 42 Fibrosis, squamous epithelium
28-6 PTCT CD140-tdTomato+ 11 5×105 Hydrogel SC (Rear) No N/a 42 N/a
ss020513_1 PTCT CD140- CD49f+ 1 Cobblestone, no foci 4 102d 3 4×105 Hydrogel SC (Shoulder) No 5.3 ± 0.6 32 Glandular, squamous epithelium, CK8/18 positive
ss020513_1 PTCT CD140- CD49f+ 242d 3 1×106 Matrigel SC (Flank) Yes 6.0 ± 0.4 10
ss020513_2 PTCT CD140- CD49f+ 1 Cobblestone, no foci 4 3 8×105 Matrigel SC (Flank) Yes 6.2 ± 1.2 10
ss082112_PTCT CD140- 6 Cobblestone, no foci 4 59d 10 1×106 Matrigel MG Yes <1 mm 10.6 Normal ductal epithelium
ss082112_PTCT CD140- 6 11 1×106 Matrigel SC (Rear) Yes 7.21 ± 0.2 10.6 Glandular, squamous epithelium
  1. NSG mice (n = 3-7), at various ages, were injected with cells at various passages post-transduction (PI) in hydrogel or Matrigel into mammary gland fat pads (MG) or subcutaneously (SC), with or without implanted estrogen (E) and progesterone (P) pellets. Cells were grown in the mice for up to 42 weeks and the widest diameter (±SEM) and features of growths are indicated