Table 1 pMEC were sorted to remove fibroblasts (CD140-), and some selected for expression of CD49f or tdTomato, and then transduced at various passages (P_T) with one of the lentiviral constructs PGK-Tantigen (PGKT), CMV-tdTomato (CMVT) or PGKT-CMVT (PTCT)

ss071712 PGKT

CD140-

1

Cobblestone, no foci

3

67d

6

1×10⁵

Hydrogel

MG

No

N/a

16.7

N/a

ss071712 CMVT

CD140-

6

1×10⁵

Hydrogel

MG

No

N/a

16.7

ss071712 PGKT

CD140-

1

7

74d

6

1×10⁵

Hydrogel

MG

No

N/a

15.7

N/a

ss071712 CMVT

CD140-

6

1×10⁵

Hydrogel

MG

No

N/a

15.7

27-3 PTCT

CD140-tdTomato+

1

Foci, radial outgrowth

4

30d

7

5×10⁵

Hydrogel

MG

No

<1 mm

19.5

Normal ductal epithelium

27-3 PTCT

CD140-tdTomato+

7

5×10⁵

Hydrogel

SC (Rear)

No

N/a

19.5

N/a

28-3 PTCT

CD140-

1

Elongated, some foci

4

30d

10

5×10⁵

Hydrogel

MG

No

15.3 ± 1.5

18.5

Fibrosis, vimentin positive

28-3 PTCT

CD140-

10

5×10⁵

Hydrogel

SC (Rear)

No

12.3 ± 1.1

18.5

27-1 PTCT

CD140-tdTomato+

1

Cobblestone, no foci

4

30d

11

5×10⁵

Hydrogel

MG

No

N/a

36.4

N/a

27-1 PTCT

CD140-tdTomato+

11

5×10⁵

Hydrogel

SC (Rear)

No

N/a

36.4

28-6 PTCT

CD140-tdTomato+

1

Foci, rapid proliferation

4

30d

11

5×10⁵

Hydrogel

MG

No

<1 mm

42

Fibrosis, squamous epithelium

28-6 PTCT

CD140-tdTomato+

11

5×10⁵

Hydrogel

SC (Rear)

No

N/a

42

N/a

ss020513_1 PTCT

CD140- CD49f+

1

Cobblestone, no foci

4

102d

3

4×10⁵

Hydrogel

SC (Shoulder)

No

5.3 ± 0.6

32

Glandular, squamous epithelium, CK8/18 positive

ss020513_1 PTCT

CD140- CD49f+

242d

3

1×10⁶

Matrigel

SC (Flank)

Yes

6.0 ± 0.4

10

ss020513_2 PTCT

CD140- CD49f+

1

Cobblestone, no foci

4

3

8×10⁵

Matrigel

SC (Flank)

Yes

6.2 ± 1.2

10

ss082112_PTCT

CD140-

6

Cobblestone, no foci

4

59d

10

1×10⁶

Matrigel

MG

Yes

<1 mm

10.6

Normal ductal epithelium

ss082112_PTCT

CD140-

6

11

1×10⁶

Matrigel

SC (Rear)

Yes

7.21 ± 0.2

10.6

Glandular, squamous epithelium