Fig. 4

Installation of lentivirus-transduced pig mammary epithelial cells (pMEC). a Representative images of red fluorescent terminal ductal lobular units identified in whole mounts of mammary glands injected with pMEC 8d after the cells were transduced with CMV-tdTomato, EF1α-tdTomato or PGK-tdTomato lentivirus. Tissues were harvested 3 or 5 weeks later. b Detection of tdTomato by PCR of genomic DNA from mammary glands analyzed in (a). Negative controls (Neg) are genomic DNA from the mammary glands of two untreated pigs. c Detection of tdTomato by PCR of genomic DNA from mammary glands injected with pMEC 24 h after the cells were transduced with CMV-tdTomato, EF1α-tdTomato or PGK-tdTomato lentivirus. Tissues were harvested 4 weeks later