Fig. 3

Effects of miR-9 overexpression on liver cancer cellular properties. a Relative miR-9 expression levels in SNU-449 cells after transfection with miR control or miR-9, 48 h post-transfection. b Cell growth of SNU-449 liver cancer cells transfected with miR negative control (miR-Control) or miR-9, 48 h and 72 h post-transfection. c Invasion of SNU-449 after transfection with miR negative control (miR-Control) or miR-9, 48 h post-transfection. d Soft agar colony assay in SNU-449 overexpressing miR-9 or miR-Control. e Effects of miR-9 overexpression on the number of SNU-449 liver tumor spheres. All data are represented as mean ± SE. ***P < 0.001, **P < 0.01