Fig. 6

Role of GTSE1 in cisplatin induced apoptosis. a AZ521-p, AZ521-con and AZ521-kd cells were treated with cisplatin (5 μm) for 24 h and expression of pro-apototic p53 and its downstream effectors bax and casp-3 were detected by western blotting. Untreated cells served as experimental control. GAPDH served as internal control (b) The induction of apoptosis in AZ521-p, AZ521-con and AZ521-kd as detected by flow cytometry using Annexin V-FITC apoptosis assay. The percentage of apoptotic cells (lower right quadrant) was calculated. Data are presented as the mean values of the florescent intensities from two independent experiments and error bars standard deviations (SD)