Fig. 4

M2 macrophage marker mRNA expression. (a) THP-1 macrophages were incubated during 24 h either with IL-4 and IL-13 (20 ng/ml each) or with IFN-γ 20 ng/ml and LPS 10 pg/ml and mRNA expression of M2 macrophage markers was studied by RT-qPCR and normalized by RPS9 expression. Results are expressed as means ± 1 S.D. (n = 3). (b) THP-1 macrophages were incubated with IL-4 and IL-13 (20 ng/ml each) during 24, 48 or 72 h and mRNA expression of M2 macrophage markers was studied by RT-qPCR and normalized by RPS9 expression. Results are expressed as means ± 1 S.D. (n = 3). Statistical analysis was carried out with a one-way ANOVA for figure A and a two-way ANOVA for figure B, followed by a Holm-Sidak post-test. NS: not significantly different. *, ** or ***: significantly different from the corresponding control (Ctrl) respectively with p < 0.05, 0.01 or 0.001; ###: significantly different with p < 0.001. Statistical analyses were performed on log-transformed data for CD206 (b), fibronectin (b) and CCL18 (b)