Fig. 4

Effect of Nrf2 on cell proliferation and apoptosis. a After shRNA-Nrf2 (shRNA-1757 or shRNA-2019) or control shRNA (shNC) transduction, the growth of Bel-7402 cells was analyzed at different time points using the MTT assay; b After Nrf2 expression plasmid (pEFGP-Nrf2-1 or pEFGP-Nrf2-2) or mock pEGFP plasmid (pEGFP-NC) transduction, the growth of HepG2 cells was analyzed at different time points using the MTT assay; c-d Flow cytometric analysis of the effect of Nrf2 on the apoptosis of Bel-7402 cells by down-regulation of expression of Nrf2; e-f Flow cytometric analysis of the effect of Nrf2 on the apoptosis of HepG2 cells by up-regulation of expression of Nrf2. g After shRNA-Nrf2 (shRNA-1757 or shRNA-2019) or control shRNA (shNC) transduction, expression of Bcl-xL were detected by western blot in Bel-7402 cells; h After Nrf2 expression plasmid (pEFGP-Nrf2-1 or pEFGP-Nrf2-2) or mock pEGFP plasmid (pEGFP-NC) transduction, expression of Bcl-xL were detected by western blot in HepG2 cells. *P < 0.05 compared with control (Bel-7402 cells or HepG2 cells respectively) or shNC and pEGFP-NC