Fig. 7

Decreased interaction of CRIF1 and Tid1 in Jurkat cells. (a) Whole cell lysates from Jurkat and Jcam cells were subjected to Tid1 immunoprecipitation and subsequent immunoblotting with anti-Tid1 and anti-CRIF1 antibodies. Jurkat lysate immunoprecipitated with normal IgG was used as a negative control (lane 4). A fraction of Jurkat lysate was loaded as a positive control (lane 1). (b) Jurkat and Jcam cells were analyzed by PLA microscopy using primary antibodies specific for CRIF1 and Tid1 (upper panels). Green fluorescence indicates CRIF1 and Tid1 interaction in situ (white arrows). Secondary antibodies alone were used as negative controls (lower panels). Scale bars of 10 μm are also shown. (c) The number of CRIF1 and Tid1 interacting complex were counted in multiple cells to obtain average number per cell