Fig. 2

Reoviral protein accumulation and active caspase-3 levels in tumor biopsies. a Reovirus replicates in the HCT116 colon cancer cell line. HCT116 cells treated or untreated with Reolysin served as a positive and negative control for reoviral protein IHC. Brown staining indicates positive staining. b Detection of reoviral and active caspase-3 protein in a biopsy specimen obtained from a pancreatic cancer patient following Reolysin and gemcitabine therapy. Reoviral and active caspase-3 levels were detected by IHC. Low, middle, and high magnification images are displayed. Arrows denote positive brown staining representing positive reoviral and active caspase-3 staining, respectively. IHC of fat cells (far right) from the same pancreatic cancer patient tissue sample served as an internal negative control. Bar represents 75 microns. The staining score for both reoviral and active caspase-3 protein was 3+