Fig. 1

ACOX2 expression in TCGA BRCA cohort. ACOX2 is involved in the oxidation of very long chain fatty acids, VLCFA, and branched chain fatty acids, BCFA, and in the synthesis of bile-acid precursor molecules as schematically illustrated in Fig. 1a. The ACOX2 intronic variant, ACOX2-i9, is initiated just upstream of exon 10 of the full-length transcript (b). The translated protein retains the Acyl-CoA oxidase domain, and the Preoxisomal Targeting Signal, but lacks the fatty acid binding domain of the full-length protein. 1c shows a model of ACOX2-i9 aligned with 2DDH (Rat ACOX2). The template 2DDH is colored green and the model (i9) is colored cyan. The fatty acid is depicted as orange colored spheres and the FAD molecule (and water depicted as small red spheres) is depicted as ball-stick and colored by element. The difference in Log2 R(ACOX2-i9/ACOX2) Tumor – log2 R Normal in 87 tumor/normal pairs from the TCGA BRCA dataset are shown in d, see Methods for details. Values > 0 indicate that the Ratio of ACOX2-i9/ACOX2 is higher in the tumor. e shows log2 expression of ACOX2 in Normal/Tumor. ACOX2 is expressed at higher levels in the Normal sample when the log2 ratio >0. Normalized log2 RPKM expression of each exon of ACOX2 in Her2 negative background separated by ER status are shown in f, and in ER negative patients separated by Her2 status (g)