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Fig. 4 | BMC Cancer

Fig. 4

From: Estrogen receptor α enhances the transcriptional activity of ETS-1 and promotes the proliferation, migration and invasion of neuroblastoma cell in a ligand dependent manner

Fig. 4

ERα can interact with ETS-1. a-b Interaction of endogenous ERα, or ETS-1 with exogenous FLAG-ETS-1, or FLAG-ERα. FLAG-tagged ERα (a) or FLAG-tagged ETS-1 (b) or FLAG empty vector (a-b) was transfected into SH-SY5Y cells. Cell lysates were immunoprecipitated by anti-FLAG monoclonal antibody, and the precipitates were then immunoblotted with anti-ETS-1 or anti-ERα antibody. c-dIn vitro interaction of ETS-1 with ERα. Glutathione-Sepharose beads bound with GST-ERα (c), GST-ETS-1 (d) or with GST (c-d) were incubated with purified FLAG-labeled ETS-1 or ERα in the presence or absence of 100nM E2. After washing the beads, the bound proteins were eluted and subjected to SDS-PAGE and IB assays

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BMC Cancer

ISSN: 1471-2407

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