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Fig. 7 | BMC Cancer

Fig. 7

From: Pharmacological targeting of valosin containing protein (VCP) induces DNA damage and selectively kills canine lymphoma cells

Fig. 7

EER-1 treatment results in TRP53 pathway activation in canine lymphoma cells. CLBL-1 cells were cultured for 6, 12 or 24 h with or without 3 μM EER-1. a Immunoblotting analysis for phospho-TRP53 (Ser 15) and total TRP53. Representative blots are shown (upper panels), each lane represents cells from a single well. Quantitative analyses of phospho-TRP53/ total TRP53 ratios (lower panel) were done using n = 3 replicates per condition. bCdkn1a mRNA expression was analyzed by real time PCR. Data are presented as mean (columns) ± SEM (error bars). Asterisks indicate a statistically significant difference (**P < 0.01 and ***P < 0.001) compared to their respective control. The experiment was repeated three times, and representative results are shown

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BMC Cancer

ISSN: 1471-2407

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