Fig. 5

VCP inhibition induces concomitant increases in Lys48 polyubiquitinated proteins and γH2AFX. a) Immunoblotting analysis was done for Lys48 ubiquitin, γH2AFX, MAP1LC3A, SQSTM1and DDIT3 on CLBL-1 cells treated on a time course with 3 μM EER-1. Representative blots are shown, each lane represents cells collected from a single well. b) Quantitative analyses of were done using n = 3 replicates per condition and normalized to ACTB (β actin) as a loading control. Data are presented as means (columns) ± SEM (error bars). Asterisks indicate a statistically significant difference (*P < 0.05, **P < 0.01 and ***P < 0.001) compared to their respective control. Veh = Vehicle. The experiment was repeated three times, and a representative result is shown