Table 1 Genes and primers for RT-qPCR
From: MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells
Gene | Primer name | Sequence (from 5’ to 3’) |
|---|---|---|
U6 | Reverse transcription | CGCTTCACGAATTTGCGTGTCAT |
| Â | Sense | GCTTCGGCAGCACATATACTAAAA |
| Â | Anti sense | GCTTCGGCAGCACATATACTAAAAT |
miR-223 | Reverse transcription | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTGGGGT |
| Â | Sense | GGGTGTCAGTTTGTCAAAT |
| Â | Anti sense | TGCGTGTCGTGGAGTC |
GAPDH | Sense | GCACCGTCAAGGCTGAGAAC |
| Â | Anti sense | TGGTGAAGACGCCAGTGGA |
MAFB | Sense | TTGTAACCAGAATCACCCTGAGGTC |
| Â | Anti sense | CCAGGGTCAGGGATGGCTAA |