Fig. 1

TRIM8 is downregulated in gliomas and affects cell proliferation. (a-c) Box-plots of TRIM8 tissues expression (a, c) and TRIM8 cells expression (b) between tumor grades. qPCR was performed to measure the level of TRIM8 transcript in a total of 70 glioma tissues and cell lines (CSS) and 378 glioma tissues specimens from TCGA LGG dataset. Kaplan-Meier curves for Overall Survival (d) and Progression-Free-Survival (e) according to TRIM8 tertiles of expression levels in patients with tumor grade III (TCGA data). (f-g) The effect of TRIM8 expression on cell proliferation was assessed by MTT assay in U87MG glioblastoma cell lines (f) and in patient glioma cell lines (g) transfected with pcDNA3 vector expressing TRIM8 or empty vector. The Y-axis represents the absorbance value. (h-j) Effect of HA-TRIM8 expression and empty vector on in vitro clonogenic potential of U87MG cells (h) and three patient glioblastoma cell lines, GBM3, GBM6, and GBM19 (j). Results are reported as mean percentage of clones from three independent experiments, and error bars represent standard deviations. These experiments were repeated three independent times and similar results were obtained each time