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Fig. 2 | BMC Cancer

Fig. 2

From: The combination of methylsulfonylmethane and tamoxifen inhibits the Jak2/STAT5b pathway and synergistically inhibits tumor growth and metastasis in ER-positive breast cancer xenografts

Fig. 2

Combination MSM-Tam treatment synergistically inhibited the Jak2/STAT5b signaling pathway. (a) Western blotting analysis of cytoplasmic protein levels in MCF-7 and T47D cells, and after treatment with Tam, MSM, or their combination for 24 h. (b) Nuclear protein level analysis in MCF-7 and T47D cells, and after treatment with Tam, MSM, or the drug combination for 24 h using western blotting. (c) The DNA binding activity of STAT5b was inhibited by the drug combination, as analyzed by a gel shift assay in MCF-7 cells. (d) The DNA binding activity of STAT5b was inhibited by the drug combination and confirmed by a ChIP assay in MCF-7 cells. Statistical analyses were conducted using the ANOVA test (**P < 0.01 and ***P < 0.001)

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BMC Cancer

ISSN: 1471-2407

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