Fig. 4

Ectopic miR-217 expression inhibits invasion of SW480 and SW620 cells. (a) Transwell method was applied to assess SW480 cells’ invasion ability after transfected with miR-217 mimics and negative control. (b) The invasiveness of SW620 cells was evaluated as mentioned above. Statistics analysis was performed by counting the stained cells that invaded to the lower chamber. All measurements were repeated three times in duplicate. (c) Western blot analysis showed the expression levels of invasion related molecules MMP2 and MMP9, cell cycle related protein cyclinD1, and apoptosis associated protein Bax and Bcl-2 after overexpression of miR-217.**P < 0.01