Fig. 1

The effects of REG4 overexpression and the recombinant protein on ovarian cancer cells. Expression levels of REG4 mRNA and protein in ovarian cancer cell lines (CAOV3, ES-2, HO8910, HO8910-PM, OVCAR3, SKOV3 and SKOV3/DDP) were screened by (a, n = 3) real-time RT-PCR and (b, respectively) Western blotting. After treatment with the recombinant protein (50 nM), growth of SKOV3 cells was significantly increased (c, n = 3), and ectopic REG4 overexpression in SKOV3 cells was observed by (d, n = 3) real-time RT-PCR and (e, respectively) Western blotting. The treatment with the REG4-expressing plasmid or the recombinant protein enhances (f, n = 3) cell proliferation, compared with mock or control cells. Results are expressed as mean ± SD of three separate experiments; *P < 0.05