Fig. 6
From: Tumor-induced loss of mural Connexin 43 gap junction activity promotes endothelial proliferation
Cx43 deficiency results in enhanced angiogenesis and decreased stabilization but does not affect tumor growth. Eo771 cells were implanted into the mammary fat pad of female mice that were wild-type (‘WT’) or heterozygous (‘Cx43’) for Cx43. a. Representative tumor sections stained for CD31 (red) shown at 20x. Bottom panel represents quantification of average CD31+ area per field (n = 8 tumors/group). *p < 0.05. b. Representative tumor sections stained for NG2 (green) and CD31 (red). Bottom panel represents quantification of relative area of NG2 positivity of CD31 positive vessels (5 fields/tumor, n = 3 tumors per group); **p < 0.01. c. Tumors were measured using calipers and volume estimated using the formula (((L + W)/2)^3)/2. d. Tumor mass was determined at the time of sacrifice (n = 10-11)