Fig. 4
From: Tumor-induced loss of mural Connexin 43 gap junction activity promotes endothelial proliferation
Mural cell inhibition of endothelial cell proliferation requires mural cell Cx43 and GJ communication. Labeled mural cells (a and b, C3H 10 T1/2 cells, or c and d, vSMC) nucleofected with control non-targeting or Cx43 siRNA were cultured with labeled HUVEC and effects on HUVEC proliferation assessed by cell counting followed by flow cytometry at indicated days. a. Pooled data from three independent experiments performed in triplicate using HUVEC from two different donors. (*p < 0.05). b. Western analysis of Cx43 expression from representative experiment from A. c. Pooled data from five independent experiments performed in triplicate using vSMC from three different donors and HUVEC from two different donors (*p < 0.05; *** p = 0.001). d. Western analysis of Cx43 expression from representative experiment from C. e. Labeled mural cells were cultured with GFP-HUVEC for four days in the presence of GAP26 blocking peptide or control scrambled peptide and effects on HUVEC proliferation assessed as in A. Data shown are from a representative of two experiment performed in triplicate (*p < 0.05)