Fig. 4
Validation of expression of HPSE2 and MMP11 using RT-qPCR. Four samples (C282, C421, C734, and N114) were picked from the training set, representing Luminal A, Luminal B, Triple negative, and normal tissue respectively. Fold changes of gene expression were calculated with the 2-ΔΔCT method, using β-actin as the house keeping gene. Results were shown as mean ± SEM from triplicates (n = 3). *p < 0.05 compared with N114, **p < 0.001 compared with N114