Fig. 6

Effects of SLC1A1 siRNA on cell death and survival parameters after chemotherapy treatment. Drug-resistant HCT116 and LoVo cell lines were transfected with siRNA against SLC1A1 or corresponding mock siRNA (siCtrl.). 24 h later, cells were exposed to SN38 (0.8 μM), oxaliplatin (20 μM) as indicated, for 24 h. Equal amounts of protein per lane were separated by SDS-PAGE and the protein level of SLC1A1 and p53 was determined by Western blotting. a Representative Western blots, with p150 as loading control. b Densitometric quantifications of relative p53 protein level, based on 3 independent experiments per condition. Data are means with S.E.M. error bars of 3 independent experiments. *) p < 0.05, **) p < 0.01, ***) p < 0.001,****) p < 0.0001 compared to the control group without drug or TBOA treatment; Two-way ANOVA with Tukey post-test