Fig. 4
Association of Cell surface GRP78 with α2M* activates c-Src and its downstream signaling pathways. (a) Western blot analysis of the expression levels of c-Src and pY416-Src in serum starved QGY-7703 and PLC cells treated with vehicle and α2M*. (b) Cell adhesion analysis of the effect of PP2 pretreatment on the binding ability of serum starved QGY-7703 cells to fibronectin upon α2M* stimulation. (c) Transwell analysis of the effect of PP2 pretreatment on the invasion of serum starved QGY-7703 cells upon α2M* stimulation. (d) Cell adhesion analysis of the effect of PP2 pretreatment on the binding ability of serum starved PLC cells to fibronectin upon α2M* stimulation. (e) Transwell analysis of the effect of PP2 pretreatment on the invasion of serum starved PLC cells upon α2M* stimulation. (f) Western blot analysis of the phosphorylation status of c-Src downstream signaling pathways in serum starved QGY-7703 and PLC cells treated with vehicle and α2M* (g) Western blot analysis of the effect of antibody blocking on the phosphorylation levels of c-Src at Y416 and FAK at Y397 in serum starved QGY-7703 and PLC cells. These experiments were repeated for 3 times in triplicate. The data were presented as \( \overline{X} \pm \mathrm{S}\mathrm{D} \) and analyzed using student’s t-test. The difference is regarded to be statistically significant when p < 0.05. *represented that the difference is statistically significant