Fig. 2

α2M* induced GRP78 translocation from cytosol to cell surface in HCC. (a) In cell western analysis of cell surface GRP78 and total GRP78 in serum starved QGY-7703 and PLC cells treated with vehicle or α2M*. (b) Schematic show of cell surface GRP78 expression in serum starved QGY-7703 and PLC cells treated with vehicle or α2M*. The experiments were repeated for 3 times in triplicate. The data were presented as \( \overline{X} \pm \mathrm{S}\mathrm{D} \) and analyzed using student’s t-test. The difference is regarded to be statistically significant when p < 0.05. * represented that the difference is statistically significant. (c) RT-PCR analysis of GRP78 mRNA levels in serum starved QGY-7703 and PLC cells treated with vehicle or α2M*. (d), Western blot analysis of exogenous and endogenous GRP78 in serum starved QGY-7703 cells transfected with GRP78-EGFP or pEGFP-N1. (e) Confocal microscopy observation of GRP78 translocation from cytosol to cell surface in serum starved QGY-7703 cells with or without α2M* stimulation. Scale Bar 25 μm