Figure 8
From: Enhancement of hypoxia-activated prodrug TH-302 anti-tumor activity by Chk1 inhibition
Effects of AZD7762 and TH-302 on pharmacodynamic biomarkers in HT29 xenografts. (A) schematic diagram illustrating the study design. (B) representative images of γH2AX, Caspase 3 and Chk1-S345 immunostainings, respectively. Insets in γH2AX images, magnification of γH2AX positive cells showing the staining pattern was either pan-nuclear or foci-formation. (C, D and E), morphometric analysis of γH2AX, Caspase 3 and Chk1-S345 positive cells. Scale bar, 100 μm; Each bar represents mean ± SEM of 5 animals per group. * p <0.05 vs. vehicle group; a, p <0.05 vs. AZD7762 group; b, p <0.05 vs. TH-302 group. #, p <0.05 vs. TAA group.