Figure 2

Effects of HBx on the expression of AFPR, AFP and Src in human normal liver cell line, L-02 cells. A, L-02 cells were transfected with pcDNA3.1-HBx vector for 72 hours, expression of HBx in L-02 cells were evidenced by Western blotting, human hepatoma cell line, PLC/PRF/5 cells were used as a positive control. B, L-02 cells were transfected with pcDNA3.1-HBx vectors for 0, 7, 14, 21 or 28 days respectively, expression of AFPR, AFP and Src in L-02 cells were evidenced by Western blotting, right column images represented the protein densitometry value contrast to internal control β-actin ratio. **P <0.01 vs control and 0 day treated groups. The images were representation of three reduplicate experiments.