Figure 7

Single cell mutation detection after whole genome amplification. Sanger sequencing traces from SKBR3 cells show single nucleotide mutations in the TP53 gene. Shown above is the region of the gene containing the p.R175H mutation from PCR products derived from single cells spiked into blood, picked and amplified. CGC encodes the wild-type arginine and CAC encodes the mutant histidine found in SKBR3 cells. The nucleotides encoding these amino acids are underlined in the traces pictured above. (A) Wild type sequence from a WBC, (B) Mutant sequence from SKBR3 cells, (C) Mixture of mutant and wild type sequence most likely due to a contaminating WBC picked along with a SKBR3 cell.