Figure 5

URG4/URGCP enhances the angiogenic capacity of HCC cells via activating the NF-κB pathway. URG4/URGCP-overexpressing HCC cells were transfected with a non-degradable mutant IκBα protein, which acts as a specific NF-κB inhibitor. A. Luciferase reporter assay of NF-κB transcriptional activity in the indicated cells. B. Representative images (left) and quantification (right) of tube-like structures formed by HUVECs on Matrigel-coated plates in the presence of CM from the indicated cells. C. Representative images (left) and quantification (right) of the number of migrated HUVEC cells in the Transwell migration assay after incubation in CM derived from the indicated cells. D. Representative images (left) and quantification (right) of neovessels formed in the CAM assay when stimulated by CM derived from the indicated cells. Data is mean ± SD of three independent experiments; *P < 0.05.