Silencing of ezrin in MES-MM98 cells. A western blot analysis of ezrin protein expression. B. In vivo time course XCelligence analysis of migrating H226 cells after 24 h from mock (control) and ezrin-specific siRNA treatment C. Wound healing assay with MPM cells after 72 hours from treatment with control siRNA (siCTRL), and ezrin-specific siRNA (siEZRIN). The histogram depicts cell migration, calculated as percentage of wound healing after 24 h from the scratch In the text: “We demonstrated that the down-regulation of ezrin slightly reduced cell viability after 24, 48, 72 hours (p < 0.001) and impinged on the migration ability of MPM cells (Figure 4 B,C,D).