Figure 2From: Mesothelioma patient derived tumor xenografts with defined BAP1 mutations that mimic the molecular characteristics of human malignant mesotheliomaBAP1 alterations in primary mesothelioma cells. Chromatograms showing BAP1 mutations (A). Arrow in the top panel indicates the location of mutation. NCI-Meso16 cells had a splice site mutation in intron 4 of BAP1. NCI-Meso17 and NCI-Meso21 had frame shift mutations at the intron15/exon16 junction and in exon 13 of BAP1 gene, respectively. Immunohistochemistry (B) showing lack of expression of BAP1 protein in cells with mutant BAP1. Only NCI-Meso19 cells which were BAP1 wild-type expressed the BAP1 protein. Western blot (C) showing the expression of BAP1 in primary mesothelioma cells. For western blot, mesothelioma cell lines NCI-H28 and NCI-H2052 were used as controls for mutant and wild-type BAP1 respectively. A faint BAP1 band was observed for NCI-Meso17 cells, which exhibits a 5-bp deletion in exon 13. Western blot showing (D) the expression of BAP1 in late passage primary mesothelioma cells. The faint BAP1 band which was observed for NCI-Meso17 cells in early passage was absent in late passage.Back to article page