Figure 3
The mechanism of GRP78-mediated GMBP1 internalization into SGC7901/ADR and SGC7901/VCR cells. (A, B): a, i: FITC-GMBP1 observed on the cell surface and in the cytoplasm. b, j: Alexa Fluor 594-transferrin observed primarily in the cytoplasm. e, m: Internalization of FITC-GMBP1 was strongly decreased after blocking the uptake of Alexa Fluor 594-transferrin. f, n: Chlorpromazine largely blocked the uptake of Alexa Fluor 594-transferrin. c, g, k, o: Nuclear staining with 4, 6-diamidino-2-phenylindole (DAPI; blue). d, h, l, p: Merged images showing the relationship between GMBP1 and transferrin.