Figure 6

Mitochondrial ND6 gene nonsense and missense mutation increases the activity of AKT and ERK/MAPK. AKT and ERK1/ERK2 expression level was determined by western blot. (A) Representative graphs of total and phosphate AKT and ERK1/ERK2 expression of A549 cells with the wild type mND6 (normal), cells with missense mutation and nonsense mutations of mitND6 gene and cells treated with rotenone. (B) Histogram showed the relative total and phosphate AKT and ERK1/ERK2 expression level of cells Data represent mean ± SEM from 4 independent experiments; *, P < 0.05; **, P < 0.01.