Proteins with differential expression/phosphorylation after AMT in A375m2 cells (Kinexus). Phosphorylation of cofilin2 at Ser3, expression of β-catenin, phosphorylation of PKCα at Ser657 and expression of PKCα were detected using corresponding antibodies in cell lysates from A375m2 strain upon AMT (induced by ROCK kinase inhibitor Y37632) both in 3D and 2D environment. Representative immunoblots are shown. Densitometric analysis was made using ImageJ software. Quantification was made from three independent experiments and normalized to that of untreated cells.