Figure 3

Ad/AFP-Casp-AFP-amiR inhibited expression of DNA polymerases in HCC cell lines. A: Ad/AFP-Casp-AFP-amiR inhibited mRNA expression of DNA polymerases in HCC cell lines. DNA polymerase α, δ, ε and β-actin mRNA levels of HCC cells HepG2, Hep3B and normal liver cell HL7702 were quantified by fluorescent real time quantitative PCR after infected by two adenoviruses with MOI 50 respectively for 48 h. β-Actin was used as the internal control to calculate the relative mRNA levels of DNA polymerase α, δ, ε. The relative mRNA level of blank control was set as 100%. *P < 0.05, **P < 0.01, ***P < 0.001, n = 3. B: Ad/AFP-Casp-AFP-amiR inhibited protein expression of DNA polymerases in HCC cell lines. DNA polymerase α, δ, ε and β-actin protein levels of HCC cells HepG2, Hep3B and normal liver cell HL7702 were monitored by Western blot after infected by two adenoviruses with MOI 50 respectively for 48 h. C: The grey values of the DNA polymerase α, δ, ε and β-actin were calculated by AlphaVIEW SA software. β-Actin was used as the internal control to calculate the relative protein levels of DNA polymerase α, δ, ε. The relative mRNA level of blank control was set as 100%. *P < 0.05, **P < 0.01, ***P < 0.001, n = 3.