Figure 4

GF induces apoptosis in serum-free medium. (A and B) Effects of 10 μM GF or 20 mM Z-VAD-fmk on cell cycle in LNCaP cells after 24 h and 48 h treatment. Cytometric analyses were performed using PI staining. (C) Western blot showed that the activation of caspase 3, 7, 9 and PARP protein in LNCaP cells treated with 10 μM GF or 20 mM Z-VAD-fmk for different time. *Significant (P <0.05); **highly significant (P <0.001).